… Body temperature was maintained using an infrared heat lamp and monitored with a rectal thermometer, and vital signs were monitored using a MouseOx pulse oximeter (STARR Lifesciences, USA). 2.4. Sample delivery. Owing … Particles suspended in the air are inhaled during normal respiration and unless cleared by airway defences, such as the mucociliary transit […]
West Nile virus (WNV) disease can be fatal for high-risk patients. Since WNV or its antigens have been identified in multiple anatomical locations of the central nervous system of persons or rodent models, one cannot know where to investigate the actual mechanism of mortality without careful studies in animal models. In this study, depressed respiratory […]
Innate lymphoid cells (ILCs), a heterogeneous cell population, are critical in orchestrating immunity and inflammation in the intestine, but whether ILCs influence immune responses or tissue homeostasis at other mucosal sites remains poorly characterized. Here we identify a population of lung-resident ILCs in mice and humans that expressed the alloantigen Thy-1 (CD90), interleukin 2 (IL-2) […]
Airway mucus is a hallmark of respiratory syncytial virus (RSV) lower respiratory tract illness. Laboratory RSV strains differentially induce airway mucus production in mice. Here, we tested the hypothesis that RSV strains differ in pathogenesis by screening six low-passage RSV clinical isolates for mucogenicity and virulence in BALB/cJ mice. The RSV clinical isolates induced variable […]
Pulmonary complications are frequent following hematopoietic stem cell transplantation, including infections and pneumonitis. We have used a murine bone marrow transplant (BMT) model and murine gammaherpesvirus, γHV-68, to study alterations in anti-viral immunity post-transplant. When challenged with γHV-68, BMT mice have reduced ability to control lytic viral replication in the lung, despite immune reconstitution. By […]
In animal models of influenza, systemic weight loss is the primary indicator of morbidity from infection, which does not assess local lung pathology or the immune response. Here, we used a mouse-adapted pulse-oximeter as a non-invasive clinical readout of lung function during influenza infection in mice, and found direct correlations between oxygen saturation levels and […]